mrfp lact c2 lactadherin c2 lact c2 Search Results


lact c2 gfp 14  (Addgene inc)
90
Addgene inc lact c2 gfp 14
Lact C2 Gfp 14, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lact c2 gfp  (Addgene inc)
93
Addgene inc lact c2 gfp
Lact C2 Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-rufy1 antibody  (Merck KGaA)
90
Merck KGaA anti-rufy1 antibody
Anti Rufy1 Antibody, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lactc2 gfp  (Addgene inc)
95
Addgene inc lactc2 gfp
Lactc2 Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid pmal c2  (New England Biolabs)
99
New England Biolabs plasmid pmal c2
Plasmid Pmal C2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dominant negative mfge 8  (Addgene inc)
93
Addgene inc dominant negative mfge 8
Dominant Negative Mfge 8, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcdna3 1 halotag7 lact c2 aaa  (Addgene inc)
96
Addgene inc pcdna3 1 halotag7 lact c2 aaa
(a) Alignment of the amino acid sequence of caspase-3 IDR, caspase-7 IDR, IDR mutants. Acidic amino acids are shown in red. Basic amino acids are shown in blue. Caspase recognition sites are shown in boxes. Mutated amino acids are highlighted in gray. (b, c) Basic and hydrophobic (BH)-scores of caspase-3 and caspase-7. The red line indicates a threshold of 0.6. Caspase-3 IDR and caspase-7 IDR are masked with orange and green blocks, respectively. (d) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 45 cells), caspase-7 KO (n = 37 cells), with 7IDR-3-FLAG (n = 35 cells), 7IDR ED/A -3-FLAG (n = 17 cells), 7IDR KR/A -3-FLAG (n = 34 cells). (e) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 15 cells), caspase-7 KO (n = 44 cells), with 7IDR-3-FLAG (n = 24 cells), 7IDR D23A -3-FLAG (n = 31 cells). (f) A schematic image illustrating differential interference contrast (DIC), TIRF, and epifluorescence observation (top). Representative images of mScarlet3 expressing HeLa cells (bottom). Scale bar: 50 μm. (g) The ratio of TIRF fluorescence intensity to epifluorescence intensity, normalized to mScarlet3 (mS3) alone. (h) Lipid overlay assays with recombinant Lact-C2-FLAG, 7IDR D23A -3 CtoG -FLAG, 7IDR D23A, ED/A - 3 CtoG -FLAG, 7IDR Δ23 -3 CtoG -FLAG, and 7IDR Δ23, KR/A -3 CtoG -FLAG. (i) A schematic diagram depicting Lact-C2 interacting with phosphatidylserine (PS) in the inner leaflet of the PM (top). Representative images of Lact-C2 fused with <t>HaloTag7</t> (bottom). Scale bars: 10 μm. (j) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 27 cells), caspase-7 KO (n = 19 cells), with 7IDR-3-FLAG (n = 16 cells), 7IDR-3-FLAG + HaloTag7-Lact-C2 (n = 18 cells), 7IDR-3-FLAG + HaloTag7-Lact-C2 AAA (n = 26 cells). (k) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 27 cells), with HaloTag7-Lact-C2 (n = 29 cells), HaloTag7-Lact-C2 AAA (n = 25 cells). For all figures, dot plots show the mean ± SEM. Statistical analysis was performed using one-way ANOVA with Dunnett’s comparison test. NS: P > 0.05; *: P < 0.05; **: P < 0.01; ****: P < 0.0001.
Pcdna3 1 Halotag7 Lact C2 Aaa, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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pegfp lact c2 plasmid  (Addgene inc)
93
Addgene inc pegfp lact c2 plasmid
(a) Alignment of the amino acid sequence of caspase-3 IDR, caspase-7 IDR, IDR mutants. Acidic amino acids are shown in red. Basic amino acids are shown in blue. Caspase recognition sites are shown in boxes. Mutated amino acids are highlighted in gray. (b, c) Basic and hydrophobic (BH)-scores of caspase-3 and caspase-7. The red line indicates a threshold of 0.6. Caspase-3 IDR and caspase-7 IDR are masked with orange and green blocks, respectively. (d) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 45 cells), caspase-7 KO (n = 37 cells), with 7IDR-3-FLAG (n = 35 cells), 7IDR ED/A -3-FLAG (n = 17 cells), 7IDR KR/A -3-FLAG (n = 34 cells). (e) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 15 cells), caspase-7 KO (n = 44 cells), with 7IDR-3-FLAG (n = 24 cells), 7IDR D23A -3-FLAG (n = 31 cells). (f) A schematic image illustrating differential interference contrast (DIC), TIRF, and epifluorescence observation (top). Representative images of mScarlet3 expressing HeLa cells (bottom). Scale bar: 50 μm. (g) The ratio of TIRF fluorescence intensity to epifluorescence intensity, normalized to mScarlet3 (mS3) alone. (h) Lipid overlay assays with recombinant Lact-C2-FLAG, 7IDR D23A -3 CtoG -FLAG, 7IDR D23A, ED/A - 3 CtoG -FLAG, 7IDR Δ23 -3 CtoG -FLAG, and 7IDR Δ23, KR/A -3 CtoG -FLAG. (i) A schematic diagram depicting Lact-C2 interacting with phosphatidylserine (PS) in the inner leaflet of the PM (top). Representative images of Lact-C2 fused with <t>HaloTag7</t> (bottom). Scale bars: 10 μm. (j) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 27 cells), caspase-7 KO (n = 19 cells), with 7IDR-3-FLAG (n = 16 cells), 7IDR-3-FLAG + HaloTag7-Lact-C2 (n = 18 cells), 7IDR-3-FLAG + HaloTag7-Lact-C2 AAA (n = 26 cells). (k) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 27 cells), with HaloTag7-Lact-C2 (n = 29 cells), HaloTag7-Lact-C2 AAA (n = 25 cells). For all figures, dot plots show the mean ± SEM. Statistical analysis was performed using one-way ANOVA with Dunnett’s comparison test. NS: P > 0.05; *: P < 0.05; **: P < 0.01; ****: P < 0.0001.
Pegfp Lact C2 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
pegfp lact c2 plasmid - by Bioz Stars, 2026-09
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hnrnp c1 c2  (Santa Cruz Biotechnology)
85
Santa Cruz Biotechnology hnrnp c1 c2
(a) Alignment of the amino acid sequence of caspase-3 IDR, caspase-7 IDR, IDR mutants. Acidic amino acids are shown in red. Basic amino acids are shown in blue. Caspase recognition sites are shown in boxes. Mutated amino acids are highlighted in gray. (b, c) Basic and hydrophobic (BH)-scores of caspase-3 and caspase-7. The red line indicates a threshold of 0.6. Caspase-3 IDR and caspase-7 IDR are masked with orange and green blocks, respectively. (d) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 45 cells), caspase-7 KO (n = 37 cells), with 7IDR-3-FLAG (n = 35 cells), 7IDR ED/A -3-FLAG (n = 17 cells), 7IDR KR/A -3-FLAG (n = 34 cells). (e) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 15 cells), caspase-7 KO (n = 44 cells), with 7IDR-3-FLAG (n = 24 cells), 7IDR D23A -3-FLAG (n = 31 cells). (f) A schematic image illustrating differential interference contrast (DIC), TIRF, and epifluorescence observation (top). Representative images of mScarlet3 expressing HeLa cells (bottom). Scale bar: 50 μm. (g) The ratio of TIRF fluorescence intensity to epifluorescence intensity, normalized to mScarlet3 (mS3) alone. (h) Lipid overlay assays with recombinant Lact-C2-FLAG, 7IDR D23A -3 CtoG -FLAG, 7IDR D23A, ED/A - 3 CtoG -FLAG, 7IDR Δ23 -3 CtoG -FLAG, and 7IDR Δ23, KR/A -3 CtoG -FLAG. (i) A schematic diagram depicting Lact-C2 interacting with phosphatidylserine (PS) in the inner leaflet of the PM (top). Representative images of Lact-C2 fused with <t>HaloTag7</t> (bottom). Scale bars: 10 μm. (j) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 27 cells), caspase-7 KO (n = 19 cells), with 7IDR-3-FLAG (n = 16 cells), 7IDR-3-FLAG + HaloTag7-Lact-C2 (n = 18 cells), 7IDR-3-FLAG + HaloTag7-Lact-C2 AAA (n = 26 cells). (k) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 27 cells), with HaloTag7-Lact-C2 (n = 29 cells), HaloTag7-Lact-C2 AAA (n = 25 cells). For all figures, dot plots show the mean ± SEM. Statistical analysis was performed using one-way ANOVA with Dunnett’s comparison test. NS: P > 0.05; *: P < 0.05; **: P < 0.01; ****: P < 0.0001.
Hnrnp C1 C2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 85 stars, based on 1 article reviews
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lact c2  (Addgene inc)
93
Addgene inc lact c2
(a) Alignment of the amino acid sequence of caspase-3 IDR, caspase-7 IDR, IDR mutants. Acidic amino acids are shown in red. Basic amino acids are shown in blue. Caspase recognition sites are shown in boxes. Mutated amino acids are highlighted in gray. (b, c) Basic and hydrophobic (BH)-scores of caspase-3 and caspase-7. The red line indicates a threshold of 0.6. Caspase-3 IDR and caspase-7 IDR are masked with orange and green blocks, respectively. (d) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 45 cells), caspase-7 KO (n = 37 cells), with 7IDR-3-FLAG (n = 35 cells), 7IDR ED/A -3-FLAG (n = 17 cells), 7IDR KR/A -3-FLAG (n = 34 cells). (e) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 15 cells), caspase-7 KO (n = 44 cells), with 7IDR-3-FLAG (n = 24 cells), 7IDR D23A -3-FLAG (n = 31 cells). (f) A schematic image illustrating differential interference contrast (DIC), TIRF, and epifluorescence observation (top). Representative images of mScarlet3 expressing HeLa cells (bottom). Scale bar: 50 μm. (g) The ratio of TIRF fluorescence intensity to epifluorescence intensity, normalized to mScarlet3 (mS3) alone. (h) Lipid overlay assays with recombinant Lact-C2-FLAG, 7IDR D23A -3 CtoG -FLAG, 7IDR D23A, ED/A - 3 CtoG -FLAG, 7IDR Δ23 -3 CtoG -FLAG, and 7IDR Δ23, KR/A -3 CtoG -FLAG. (i) A schematic diagram depicting Lact-C2 interacting with phosphatidylserine (PS) in the inner leaflet of the PM (top). Representative images of Lact-C2 fused with <t>HaloTag7</t> (bottom). Scale bars: 10 μm. (j) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 27 cells), caspase-7 KO (n = 19 cells), with 7IDR-3-FLAG (n = 16 cells), 7IDR-3-FLAG + HaloTag7-Lact-C2 (n = 18 cells), 7IDR-3-FLAG + HaloTag7-Lact-C2 AAA (n = 26 cells). (k) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 27 cells), with HaloTag7-Lact-C2 (n = 29 cells), HaloTag7-Lact-C2 AAA (n = 25 cells). For all figures, dot plots show the mean ± SEM. Statistical analysis was performed using one-way ANOVA with Dunnett’s comparison test. NS: P > 0.05; *: P < 0.05; **: P < 0.01; ****: P < 0.0001.
Lact C2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pmal c2  (New England Biolabs)
96
New England Biolabs pmal c2
(a) Alignment of the amino acid sequence of caspase-3 IDR, caspase-7 IDR, IDR mutants. Acidic amino acids are shown in red. Basic amino acids are shown in blue. Caspase recognition sites are shown in boxes. Mutated amino acids are highlighted in gray. (b, c) Basic and hydrophobic (BH)-scores of caspase-3 and caspase-7. The red line indicates a threshold of 0.6. Caspase-3 IDR and caspase-7 IDR are masked with orange and green blocks, respectively. (d) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 45 cells), caspase-7 KO (n = 37 cells), with 7IDR-3-FLAG (n = 35 cells), 7IDR ED/A -3-FLAG (n = 17 cells), 7IDR KR/A -3-FLAG (n = 34 cells). (e) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 15 cells), caspase-7 KO (n = 44 cells), with 7IDR-3-FLAG (n = 24 cells), 7IDR D23A -3-FLAG (n = 31 cells). (f) A schematic image illustrating differential interference contrast (DIC), TIRF, and epifluorescence observation (top). Representative images of mScarlet3 expressing HeLa cells (bottom). Scale bar: 50 μm. (g) The ratio of TIRF fluorescence intensity to epifluorescence intensity, normalized to mScarlet3 (mS3) alone. (h) Lipid overlay assays with recombinant Lact-C2-FLAG, 7IDR D23A -3 CtoG -FLAG, 7IDR D23A, ED/A - 3 CtoG -FLAG, 7IDR Δ23 -3 CtoG -FLAG, and 7IDR Δ23, KR/A -3 CtoG -FLAG. (i) A schematic diagram depicting Lact-C2 interacting with phosphatidylserine (PS) in the inner leaflet of the PM (top). Representative images of Lact-C2 fused with <t>HaloTag7</t> (bottom). Scale bars: 10 μm. (j) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 27 cells), caspase-7 KO (n = 19 cells), with 7IDR-3-FLAG (n = 16 cells), 7IDR-3-FLAG + HaloTag7-Lact-C2 (n = 18 cells), 7IDR-3-FLAG + HaloTag7-Lact-C2 AAA (n = 26 cells). (k) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 27 cells), with HaloTag7-Lact-C2 (n = 29 cells), HaloTag7-Lact-C2 AAA (n = 25 cells). For all figures, dot plots show the mean ± SEM. Statistical analysis was performed using one-way ANOVA with Dunnett’s comparison test. NS: P > 0.05; *: P < 0.05; **: P < 0.01; ****: P < 0.0001.
Pmal C2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mrfp+lact+c2+lactadherin+c2+lact+c2/pMAL/10__1111_slash_j__1365___313x__2011__04772__x-208-12-18
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ptdser mrfp lact c2 biosensors  (Addgene inc)
93
Addgene inc ptdser mrfp lact c2 biosensors
(a) Alignment of the amino acid sequence of caspase-3 IDR, caspase-7 IDR, IDR mutants. Acidic amino acids are shown in red. Basic amino acids are shown in blue. Caspase recognition sites are shown in boxes. Mutated amino acids are highlighted in gray. (b, c) Basic and hydrophobic (BH)-scores of caspase-3 and caspase-7. The red line indicates a threshold of 0.6. Caspase-3 IDR and caspase-7 IDR are masked with orange and green blocks, respectively. (d) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 45 cells), caspase-7 KO (n = 37 cells), with 7IDR-3-FLAG (n = 35 cells), 7IDR ED/A -3-FLAG (n = 17 cells), 7IDR KR/A -3-FLAG (n = 34 cells). (e) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 15 cells), caspase-7 KO (n = 44 cells), with 7IDR-3-FLAG (n = 24 cells), 7IDR D23A -3-FLAG (n = 31 cells). (f) A schematic image illustrating differential interference contrast (DIC), TIRF, and epifluorescence observation (top). Representative images of mScarlet3 expressing HeLa cells (bottom). Scale bar: 50 μm. (g) The ratio of TIRF fluorescence intensity to epifluorescence intensity, normalized to mScarlet3 (mS3) alone. (h) Lipid overlay assays with recombinant Lact-C2-FLAG, 7IDR D23A -3 CtoG -FLAG, 7IDR D23A, ED/A - 3 CtoG -FLAG, 7IDR Δ23 -3 CtoG -FLAG, and 7IDR Δ23, KR/A -3 CtoG -FLAG. (i) A schematic diagram depicting Lact-C2 interacting with phosphatidylserine (PS) in the inner leaflet of the PM (top). Representative images of Lact-C2 fused with <t>HaloTag7</t> (bottom). Scale bars: 10 μm. (j) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 27 cells), caspase-7 KO (n = 19 cells), with 7IDR-3-FLAG (n = 16 cells), 7IDR-3-FLAG + HaloTag7-Lact-C2 (n = 18 cells), 7IDR-3-FLAG + HaloTag7-Lact-C2 AAA (n = 26 cells). (k) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 27 cells), with HaloTag7-Lact-C2 (n = 29 cells), HaloTag7-Lact-C2 AAA (n = 25 cells). For all figures, dot plots show the mean ± SEM. Statistical analysis was performed using one-way ANOVA with Dunnett’s comparison test. NS: P > 0.05; *: P < 0.05; **: P < 0.01; ****: P < 0.0001.
Ptdser Mrfp Lact C2 Biosensors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mrfp+lact+c2+lactadherin+c2+lact+c2/ER-mRFP+(Plasmid+%2362236)/pmc11982563-562-6-11
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Image Search Results


(a) Alignment of the amino acid sequence of caspase-3 IDR, caspase-7 IDR, IDR mutants. Acidic amino acids are shown in red. Basic amino acids are shown in blue. Caspase recognition sites are shown in boxes. Mutated amino acids are highlighted in gray. (b, c) Basic and hydrophobic (BH)-scores of caspase-3 and caspase-7. The red line indicates a threshold of 0.6. Caspase-3 IDR and caspase-7 IDR are masked with orange and green blocks, respectively. (d) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 45 cells), caspase-7 KO (n = 37 cells), with 7IDR-3-FLAG (n = 35 cells), 7IDR ED/A -3-FLAG (n = 17 cells), 7IDR KR/A -3-FLAG (n = 34 cells). (e) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 15 cells), caspase-7 KO (n = 44 cells), with 7IDR-3-FLAG (n = 24 cells), 7IDR D23A -3-FLAG (n = 31 cells). (f) A schematic image illustrating differential interference contrast (DIC), TIRF, and epifluorescence observation (top). Representative images of mScarlet3 expressing HeLa cells (bottom). Scale bar: 50 μm. (g) The ratio of TIRF fluorescence intensity to epifluorescence intensity, normalized to mScarlet3 (mS3) alone. (h) Lipid overlay assays with recombinant Lact-C2-FLAG, 7IDR D23A -3 CtoG -FLAG, 7IDR D23A, ED/A - 3 CtoG -FLAG, 7IDR Δ23 -3 CtoG -FLAG, and 7IDR Δ23, KR/A -3 CtoG -FLAG. (i) A schematic diagram depicting Lact-C2 interacting with phosphatidylserine (PS) in the inner leaflet of the PM (top). Representative images of Lact-C2 fused with HaloTag7 (bottom). Scale bars: 10 μm. (j) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 27 cells), caspase-7 KO (n = 19 cells), with 7IDR-3-FLAG (n = 16 cells), 7IDR-3-FLAG + HaloTag7-Lact-C2 (n = 18 cells), 7IDR-3-FLAG + HaloTag7-Lact-C2 AAA (n = 26 cells). (k) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 27 cells), with HaloTag7-Lact-C2 (n = 29 cells), HaloTag7-Lact-C2 AAA (n = 25 cells). For all figures, dot plots show the mean ± SEM. Statistical analysis was performed using one-way ANOVA with Dunnett’s comparison test. NS: P > 0.05; *: P < 0.05; **: P < 0.01; ****: P < 0.0001.

Journal: bioRxiv

Article Title: Early-peaking caspase-7 activity at the plasma membrane drives apoptotic phosphatidylserine exposure

doi: 10.1101/2025.03.17.643603

Figure Lengend Snippet: (a) Alignment of the amino acid sequence of caspase-3 IDR, caspase-7 IDR, IDR mutants. Acidic amino acids are shown in red. Basic amino acids are shown in blue. Caspase recognition sites are shown in boxes. Mutated amino acids are highlighted in gray. (b, c) Basic and hydrophobic (BH)-scores of caspase-3 and caspase-7. The red line indicates a threshold of 0.6. Caspase-3 IDR and caspase-7 IDR are masked with orange and green blocks, respectively. (d) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 45 cells), caspase-7 KO (n = 37 cells), with 7IDR-3-FLAG (n = 35 cells), 7IDR ED/A -3-FLAG (n = 17 cells), 7IDR KR/A -3-FLAG (n = 34 cells). (e) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 15 cells), caspase-7 KO (n = 44 cells), with 7IDR-3-FLAG (n = 24 cells), 7IDR D23A -3-FLAG (n = 31 cells). (f) A schematic image illustrating differential interference contrast (DIC), TIRF, and epifluorescence observation (top). Representative images of mScarlet3 expressing HeLa cells (bottom). Scale bar: 50 μm. (g) The ratio of TIRF fluorescence intensity to epifluorescence intensity, normalized to mScarlet3 (mS3) alone. (h) Lipid overlay assays with recombinant Lact-C2-FLAG, 7IDR D23A -3 CtoG -FLAG, 7IDR D23A, ED/A - 3 CtoG -FLAG, 7IDR Δ23 -3 CtoG -FLAG, and 7IDR Δ23, KR/A -3 CtoG -FLAG. (i) A schematic diagram depicting Lact-C2 interacting with phosphatidylserine (PS) in the inner leaflet of the PM (top). Representative images of Lact-C2 fused with HaloTag7 (bottom). Scale bars: 10 μm. (j) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 27 cells), caspase-7 KO (n = 19 cells), with 7IDR-3-FLAG (n = 16 cells), 7IDR-3-FLAG + HaloTag7-Lact-C2 (n = 18 cells), 7IDR-3-FLAG + HaloTag7-Lact-C2 AAA (n = 26 cells). (k) Δt 1/2 of PM Dual FRET transiently expressed in WT (n = 27 cells), with HaloTag7-Lact-C2 (n = 29 cells), HaloTag7-Lact-C2 AAA (n = 25 cells). For all figures, dot plots show the mean ± SEM. Statistical analysis was performed using one-way ANOVA with Dunnett’s comparison test. NS: P > 0.05; *: P < 0.05; **: P < 0.01; ****: P < 0.0001.

Article Snippet: For pcDNA3.1-HaloTag7-Lact-C2 and pcDNA3.1-HaloTag7-Lact-C2 AAA , the coding sequence of Lact-C2 was PCR-amplified using primers for Lact-C2 or mutation-introducing primers (W26A; TGG to GCC, W33A; TGG to GCC, F34A; TTT to GCC) from Lact-C2-GFP (#22852; Addgene).

Techniques: Sequencing, Expressing, Fluorescence, Recombinant, Comparison